From an organizational perspective, open innovation (OI) capability assessments are becoming increasingly important. The authors propose that an organization’s attitude toward interactive OI activities among OI stakeholders can reveal its degree of capability. This paper aims to focus on an organization’s OI attitude measurement scales and develop a framework linked to the role perspectives and loci of OI activities occurring at the organizational level. This research will introduce a practical, theory-based indication of OI assessment by combining a deductive process that identifies organizational OI attitude constructs with an inductive framework development process. First, the authors conducted an extensive literature review of attitude measurement on the execution of OI. Then, they performed empirical data analysis using a large-scale structured attitude assessment survey from individuals in domestic and multi-national corporations (n = 134), which led to the development of questionnaire sets on attitude evaluation. This study contributes to developing an organizational OI attitude assessment scale. Furthermore, based on empirical data analysis, the research framework demonstrated the reliability and validity of the organizational OI attitude measurement scale. Specifically, the scale contains proven questionnaires assessing OI attitudes by interrogating individual actors’ impact, behavior, and cognition regarding their organization’s OI activities. The organization’s three role perspectives (transfer, absorption, and brokerage) and two loci of activities in the OI ecosystem provide six distinct dimensions, suggesting areas of focus for a firm’s strategic OI direction.