The aim of this study was to detect the amount of aflatoxin total (AFT) in raw pistachio samples in Torbat-e-Heydarieh in north-east of Iran. For this purpose, 48 raw pistachio samples were collected from Karimi farm in Torbat-e-Heydarieh during October 2014 and analyzed for AFT content by enzyme linked immune-sorbent assay (ELISA) technique. Results showed that the the minimum, maximum and average ± standard deviation of AFT was respectively 0.27, 0.3, 0.95±0.62ng/g in the raw pistachio samples. The AFT concentration in the samples was lower than the national (15ng/g) and EU (10ng/g) standards. The situation seems to be suitable in the raw samples, but the storage condition must be in mind to prevent the contamination and production of the mycotoxin.