The purpose of this research is to know how much influence of entrepreneurial knowledge to student’s interest from Management major at University Catholic of St. Thomas Medan in entrepreneurship. The research uses descriptive and quantitative methods with 96 samples which taken randomly using online questionnaire and data analysis using simple linear regression. The research result shows that 51,04 % of students with entrepreneurial knowledge agree with entrepreneurship course and 44,17 % of students agree that entrepreneurial knowledge can support student’s interest in entrepreneurship. From the test result obtained: 1) Determination coefficient (R2) is equal to 0,230 which student’s interest in entrepreneurship is equal to 23,0 % influenced by entrepreneurial knowledge and 77,0 % influenced by other factors which are not explained in this research (for example: synergy of business introduction, Marketing, and e-commerce courses). 2) Regression equation: Y = 1.966+0,490+e, where level of significance 0.000 < 0,05, t-count is equal to 5.306, which is bigger than t-table that is equal to 1,65. So, Ho is rejected which means entrepreneurial knowledge has positive impact on student’s interest in entrepreneurship. Based on discussion we can conclude that: 1) entrepreneurial knowledge still has low impact on student’s interest in entrepreneurship (R2 = 0.230), hence, to develop student’s interest in entrepreneurship, it is necessary to give students more applicable courses with support from other courses like business introduction, Marketing, and e-commerce courses. 2) there is still students disinterest in entrepreneurship, hence University Catholic of Saint Thomas Medan need to establish students union as an apprenticeship place or a place to learn entrepreneurship for students with high interest in entrepreneurship and avoid them from unemployment. 3) For further research it is better to add other factors which can explain dependent variable of student’s interest in entrepreneurship like Probability, Personality, entrepreneurial motivation, family environment of students after graduated from their university.