Background: With the increase of the number of smokers, tobacco exposure among pregnant women is becoming more and more common. Pregnant women exposed to frst‑hand smoke and second‑hand smoke are susceptible to physiological and psychological health issues has been proved in previous studies. Nevertheless, there are no enough studies focus on the impact of third‑hand smoke during pregnancy. This study aimed to assess and compare health‑ related quality of life for pregnant women with exposure to frst‑hand smoke, second‑hand smoke, third‑hand smoke and non‑exposure to tobacco in mainland China. Methods: National‑based cross‑sectional study is based on a questionnaire survey which collects information including demographics, smoking behaviors and self‑evaluation. All questionnaires were delivered and collected from August to September 2019. EuroQol group’s visual analog scale and EuroQoL Five‑dimension Questionnaire were used to collect data in mainland China. Results: Totally, 15,682 pregnant women were included in this study, among which non‑exposure to smoke were 7564 (48.2%), exposed to frst‑hand smoke, second‑hand smoke and third‑hand smoke were 89 (0.6%), 2349 (15.0%), and 5680 (36.2%) respectively. Pregnant women without tobacco exposure had the highest EuroQol group’s visual analog scale score (mean value= 85.4[SD= 14.0]), while those with frst‑hand smoke had the lowest score (mean value= 77.4[SD= 22.2]). Among all fve dimensions of EuroQoL Five‑dimension Questionnaire, there were significant diferences of EQ‑index among groups with diferent tobacco exposure in usual activity and anxiety or depression dimensions (p < 0.001). Conclusions: Third‑hand smoke exposure had close relationship with low health‑related quality of life in pregnant women. Moreover, second‑hand smoke exposure signifcantly led more problems on mental dimension of pregnant women.