Dam break studies consist of two submodels: (a) the dam breach submodel which derives the flood hydrograph and (b) the hydrodynamic submodel which, using the flood hydrograph, derives the flood peaks and maximum water depths in the downstream reaches of the river. In this paper, a thorough investigation of the uncertainty observed in the output of the hydrodynamic model, due to the seven dam breach parameters, is performed in a real-world case study (Papadiana Dam, located at Tavronitis River in Crete, Greece). Three levels of uncertainty are examined (flow peak of the flood hydrograph at the dam location, flow peaks and maximum water depths downstream along the river) with two methods: (a) a Morris-based sensitivity analysis for investigating the influence of each parameter on the final results; (b) a Monte Carlo-based forward uncertainty analysis for defining the distribution of uncertainty band and its statistical characteristics. Among others, it is found that uncertainty of the flow peaks is greater than the uncertainty of the maximum water depths, whereas there is a decreasing trend of uncertainty as we move downstream along the river.