To study the influence of masonry infill walls on the hysteretic performance of reinforced concrete frames, a cyclic experiment was conducted for three two-story and two-span reinforced concrete frame structures, including one reinforced concrete frame without infill walls and two frames with infill walls. Whether the infill walls were constructed in the frames and the type of infilled material were the main parameters of the test. The major results reveal that: the infill walls clearly changed the mechanical mechanism of the frame structure at the early stage of loading,magnified the stiffness and horizontal bearing capacity of the frame structure, and enhanced the energy dissipation capacity of the frame structure, but reduced the deformation performance of the frame structure. In the later stage of loading, the infill walls would no longer work as one with the frame gradually with the failure of the infill walls, and the above performance of the structure would approach the empty frame structure. Moreover, the initial stiffness, energy dissipation capacity, and horizontal bearing capacity of the frame with infill walls of clay hollow bricks werethe highest among the three specimens. But due to the strong diagonal bracing effect, the damage to the top of the columns and beamcolumn joints was serious, the yield displacement was reduced significantly, and the shear failure of the top of the columns and the joints occurred prematurely, which showed poor performance of deformation and ductility. However, the frame with infill walls of relatively soft aerated lightweight concrete blocks showed better performance of deformation and ductility.